Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (16): 2994-3001.doi: 10.3969/j.issn.2095-4344.2013.16.021
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Guo Wei, Gong Du-hui, Hu Zhi-wei, Cheng Yuan, Gao Yi, Pan Ming-xin
Received:
2013-03-15
Revised:
2013-03-19
Online:
2013-04-16
Published:
2013-04-16
Contact:
Pan Ming-xin, Doctor, Chief physician, Professor, Second Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
pmxwxy@sohu.com
About author:
Guo Wei★, Studying for master’s degree, Physician, Second Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China
921329794@qq.com
Supported by:
he National High Technology Research and Development Program of China (863 Program), No. 2006AA02A141*; the Science and Technology Plan of Guangdong Province, No. 2007B010600009*
CLC Number:
Guo Wei, Gong Du-hui, Hu Zhi-wei, Cheng Yuan, Gao Yi, Pan Ming-xin. Preparation and characterization of hepatocyte-specific macroporous microcarrier[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2994-3001.
2.1 丝素蛋白/半乳糖基化壳聚糖大孔微载体的吸水率和密度 经冷冻干燥处理得到的丝素蛋白/半乳糖基化壳聚糖大孔微载体,完全保持了其三维网络结构, 这种结构使其容易溶胀,吸水率可达5.4 mL/g以上,随着半乳糖基化壳聚糖所占比例的增加而略有增加。通过此方法制备出的微载体结构疏松,湿态微载体密度较小,经反复多次测量,其浓度为1.02-1.05 g/mL,适合用于旋转微重力培养及搅拌培养。 2.2 丝素蛋白/半乳糖基化壳聚糖大孔微载体的形态 见图1。 图1为倒置显微镜下观察丝素蛋白/半乳糖基化壳聚糖大孔微载体:该大孔微载体为白色或淡黄色,呈半透明的球形;其内部疏松多孔,孔结构分布均匀;轻轻晃动时能清楚地观察到其三维立体结构。该大孔微载体内部疏松多孔的结构扩大了比表面积,并提供了较大的空体积,能将细胞固定在孔内生长,且便于观察,适合用于肝细胞高密度培养。"
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